Probing _ 2nd Antibodies
HRP conjugates
Antigen | 품번 | 품명 |
Mouse IgG | Mouse IgG, HRP-linked F(ab’)2 fragment (from sheep) 1 mL | NA9310-1ML |
Mouse IgG, HRP-linked whole Ab (from sheep) 100 μL | NA931-100UL | |
Mouse IgG, HRP-linked whole Ab (from sheep) 1 mL | NA931-1ML | |
Rabbit IgG | Rabbit IgG, HRP-linked F(ab’)2 fragment (from donkey) 1 mL | NA9340-1ML |
Rabbit IgG, HRP-linked Whole Ab (from donkey) 100 μL | NA934-100UL | |
Rabbit IgG, HRP-linked Whole Ab (from donkey) 1 mL | NA934-1ML | |
Rat IgG | Rat IgG, HRP-linked whole antibody (from goat) 1 mL | NA935 |
Human IgG | Human IgG, HRP-linked whole Ab (from sheep) 1 mL | NA933-1ML |
Streptavidin | Streptavidin-Biotinylated Horseradish Peroxidase Complex 2 mL | RPN1051-2ML |
Streptavidin-Horseradish Peroxidase Conjugate 100 μL | RPN1231-100UL | |
Streptavidin-Horseradish Peroxidase Conjugate 2 mL | RPN1231-2ML | |
GST | Anti-GST HRP Conjugate 75 μL | RPN1236 |
Fluorescence conjugates
•동시에 여러 타겟 단백질을 detection 가능
•항체 제거 및 reprobing 과정이 필요하지 않아 샘플 손실 방지
•안정적인 형광 시그널로 멤브레인의 보관 및 재스캔 가능
•Total protein normalization이 가능하여 신뢰성 있고 정확한 정량 가능
Antigen | 품명 | Excitation Max | Emission Max | 품번 |
Mouse IgG | ECL Plex goat-α-mouse IgG-Cy3 150 μg | 550 | 570 | PA43009 |
ECL Plex goat-α-mouse IgG-Cy5 150 μg | 649 | 670 | PA45009 | |
CyDye 700 goat-anti-mouse 0.1 mg | 678 | 690 | 29360784 | |
CyDye 700 goat-anti-mouse 0.5 mg | 678 | 690 | 29360785 | |
CyDye 800 goat-anti-mouse 0.1 mg | 774 | 790 | 29360788 | |
CyDye 800 goat-anti-mouse 0.5 mg | 774 | 790 | 29360789 | |
Rabbit IgG | ECL Plex goat-α-rabbit IgG-Cy3 150 μg | 550 | 570 | 28901106 |
ECL Plex goat-α-rabbit IgG-Cy5 150 μg | 649 | 670 | PA45011 | |
CyDye 700 goat-anti-rabbit 0.1 mg | 678 | 690 | 29360786 | |
CyDye 700 goat-anti-rabbit 0.5 mg | 678 | 690 | 29360787 | |
CyDye 800 goat-anti-rabbit 0.1 mg | 774 | 790 | 29360790 | |
CyDye 800 goat-anti-rabbit 0.5 mg | 774 | 790 | 29360791 |
*Total protein normalization
•Dark blue : Total protein, NHS ester Cy3 라벨링
•Red : ERK, CyDye 700
•Light blue : tubulin, CyDye 800