기본원리
타겟 단백질의 세포 내 위치에 따라 적합한 Fixation/Permeabilization buffer를 처리한 후,
타겟 단백질에 대한 형광 항체를 염색하여 분석합니다.
Detection Cytokine
Transport inhibitor를 처리하여 사이토카인(Cytokine)/케모카인(Chemokine)의 분비를 막은 후 분석합니다.
| 품명 | 구성품 | 용량 | 품번 |
| BD Cytofix/Cytoperm™ Solution Kit | Perm/Wash Buffer Fixation/Permeabilization Solution | 250 Tests | 554714 |
| BD Cytofix/Cytoperm™ Solution Kit with BD GolgiStop™ | Perm/Wash Buffer Fixation/Permeabilization Solution BD GolgiStop™ Protein Transport Inhibitor® | 250 Tests | 554715 |
| BD Cytofix/Cytoperm™ Solution Kit with BD GolgiPlug™ | 250 Tests | 555028 | |
| Perm/Wash Buffer | 100 mL | 554723 | |
| Fixation/Permeabilization Solution | 125 mL | 554722 | |
| BD GolgiStop™ Protein Transport Inhibitor | Monensin solution | 0.7 mL | 554724 |
| BD GolgiPlug™ Protein Transport Inhibitor | Brefeldin A solution | 1 mL | 555029 |
| BD IntraSure™ Kit | Reagent A (Fix) Reagent B (Perm) | 50 Tests | 641776 |
추천 Transport Inhibitor
| Species | Cytokines | Transport Inhibitor |
| Human | IL-1α, IL-6, IL-8, TNF-α | Monensin |
| IFN-γ, IL-2, IL-10, IL-12, MCP-1, MCP-3, MIG, MIP-1α, RANTES | Either monensin or brefeldin A | |
| Mouse | IL-6, IL-12, TNF-α | Brefeldin A |
| GM-CSF, IL-3, IL-4, IL-5, IL-10 | Monensin | |
| IFN-γ, IL-2 | Either monensin or brefeldin A |
Fastimmune
Whole Blood Assay로 빠른 분석이 가능합니다.
| 품명 | 용량 | 품번 |
| Brefeldin A Solution | 250 ul | 347688 |
| BD FACS Permeabilizing Solution 2 | 200 Tests | 347692 |
| 500 Tests | 340973 | |
| Fastimmune EDTA Solution | 2.5 mL | 347689 |
| BD Lysing Solution | 100 mL | 349202 |
Detection of Transcription Factors
FoxP3, Sox17과 같은 Transcription factor의 분석을 위한 버퍼
| 품명 | 구성품 | 용량 | 품번 |
| Transcription Factor Buffer Set | TF Fix/Perm Buffer TF Diluent Buffer TF Perm/wash Buffer | 25 Tests | 562725 |
| 100 Tests | 562574 | ||
| Human FoxP3 Buffer Set | Human FoxP3 Buffer A Human FoxP3 Buffer B | 100 Tests | 560098 |
| Mouse Foxp3 Buffer Set | Mouse Foxp3 Fixation Concentrate Mouse Foxp3 Permeabilization Concentrate | 100 Tests | 560409 |
Detection of Phosphorylated Protein
단일 세포(Single cell)가 가진 타겟 단백질의 발현 수준을 분석할 수 있으며,
타겟 단백질의 epitope 위치에 따라 버퍼를 구분하여 사용합니다.
| 품명 | 설명 | 용량 | 품번 |
| BD Phosflow™ Lyse/Fix Buffer | 250 mL | 558049 | |
| BD Phosflow™ Fix Buffer I | 250 mL | 557870 | |
| BD Phosflow™ Perm/Wash Buffer I | Mild Detergent Method | 125 mL | 557885 |
| BD Phosflow™ Perm Buffer II | Mild Alcohol Method (64.86%) | 125 mL | 558052 |
| BD Phosflow™ Perm Buffer III | Harsh Alcohol Method (87.68%) | 125 mL | 558050 |
| BD Phosflow™ Perm Buffer IV | Harsh Detergent Method | 50 mL | 560746 |
기타 Buffer
| 품명 | 설명 | 용량 | 품번 |
| BD Pharm Lyse™ Lysing Buffer (10X concentrate) | Ammonium Chloride-based Lysing Reagent | 100 mL | 555899 |
| BD Cytofix™ Fixation Buffer | 100 mL | 554655 | |
| BD Cytoperm™ Permeabilization Buffer Plus | BrdU Flow Kit용 | 10 mL | 561651 |
